Comparative Safety Evaluation of PotentoxÂ® Vs Co-Administration of Cefepime And Amikacin In Healthy Albino Rat
Aminoglycosides and cephalosporins are widely used antibacterial agents for the treatment of both severe aerobic Gram negative and Gram positive bacterial infections. Though the combination therapy scores over single antibiotic therapy in terms of efficacy but the risk of associated nephro-toxicity and hepato-toxicity is a cause of concern. Objective: The present study was undertaken to determine the comparative effect of co-administration of cefepime and amikacin one after the other verus Potentox® (single injection combination of cefepime and amikacin supplemented with chemical vector having antioxidant property) and their effect on liver and kidney functions in a healthy albino rat model. The purpose is to compare both regimens for comparative nephro and hepato-toxicity profile and effect of chemical vector, in Potentox®. Materials and Methods: Eighteen healthy albino rat were used in the experiment and divided in three groups containing six each. The respective drugs (amikacin, cefepime, Potentox®)were administered through intravenous route for 10 days. At the end of 3rd and 10th days of treatments blood samples were collected and tests were performed for catalase activity, reduced glutathion, total thiol, malonaldialdehyde, serum glutamic oxaloacetic transaminase, serum glutamic pyruvic transaminase, alkaline phosphatase, creatinine, uric acid and urea. Results: Experimental results showed that catalase activity, reduced glutathion and total thiol levels decreased significantly in cefepime followed by amikacin treated group, while no significant change occurred in Potentox® treated group. The malonaldialdehyde, serum glutamic oxaloacetic transaminase, serum glutamic pyruvic transaminase, alkaline phosphatase, creatinine, uric acid and urea levels were significantly increased in cefepime followed by amikacin treated group and this increase was not of much significance in Potentox® treated group. Conclusion: These findings reveals that the presence of chemical vector in Potentox® has yielded a significant free radical scavenging property which may contribute in decreasing the aminoglycoside induced nephrotoxicity and hepatotoxicity.